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KMID : 0374019830060010003
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Ewha Medical Journal
1983 Volume.6 No. 1 p.3 ~ p.15
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Effects of Chronic Ethanol Adninistration and Cold Exposure on Rabbit Adrenocortical 11β-Hydroxylase and 18-Hydroxylase Activities
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이덕용/Lee, Duk-Yong
성낙응/Sung, Nak-Eung
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Abstract
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Rabbit adrenocrtical mitochondria contains cytochrome P-450 dependent hydroxylase system, capable of catalyzing the hydroxylation as well as other modifications of variety of lipids and foreign compounds, including drugs, pesticides, carcinogens. Ethanol enhances the activity of the microsomal enzyme system, and cold exposure increases various enzyme activity. The present study investigated the effects of chronic ethanol administation and cold exposure on rabbit adrenocortical mitochondrial 11 .8-and 18-hydroxylase activity, cytochrome P-450 and b6 and AAF hydroxylation, serum cortisol level and lipid content of the adrenal cortex.
The results obtained are
1) When rabbits were administrated with 15% and 30% ethanol and were exposed to cold, the level of adrenocortical mitochondrial 11 P-hydroxylase activity was increased, but 18-hydroxylase activity was slightly increased only in the cold exposure group. This result corresponded with an increase of serum cortisol level.
2) When rabbits were administrated ´witin ethanol and were exposed to cold, the level of cytochrome P-450 in adrenocortical mitochondria was increased, but cytochrome bs level did not `change. Ring-hydroxylation of AAF did not change, but N-hydroxylation was increased.
3) When rabbits were administrated with ethanol and were exposed to cold, the levels of total cholesterol and triglyceride in adrenal cortex was remarkably increased, but phospholipid level was reduced.
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